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The cytoplasmic fraction was collected by centrifugation at 10,000 g for 10 min
According to Yucca's published guidance, a sealed vial with clear solution (a light pink tint from added B12 is normal for compounded formulations) and no visible particles is considered safe to use
The American College of Physicians guidelines note that continued treatment is necessary to maintain weight loss, as discontinuation often leads to weight regain
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