After FASP digestion, samples were brought up in 2 % acetonitrile, 0.1 % formic acid and separated with an EasyLC into a QE-Plus (Thermo Fisher Scientific) mass spectrometer and eluted over a 90-min gradient from 100% buffer A (2% acetonitrile, 0.1% formic acid) to 100% buffer B (90% acetonitrile, 0.1% formic acid) with an MS resolution of 70,000 and an MS2 resolution of 35,000 running a top 15 DDA method
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